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KMID : 0377519860110020135
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Chung-Ang Journal of Medicine
1986 Volume.11 No. 2 p.135 ~ p.146
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Evaluation of Antigens from Different Parts of Cysticercus cellulosae in Serological Diagnosis of Human Cysticercosis
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Cho, Bong-Kil/최봉길
Kim, Suk-Il/Kang Shin-Yong/Cho, Seung-Yull/김석일/강신영/조승열
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Abstract
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In order to evaluate the antigens which were prepared from different anatomical parts of C. cellulosae, cystic fluid, bladder wall and scolex were collected separately. The saline extracts of bladder wall. scolex and unruptured whole worms were prepared. In addition, saline ex-tract of adult T. saginata was made. These 5 antigens were tested with sera from 128 con-firmed cysticercosis, 18 sparganosis, 31 paragonimiasis, 18 taeniasis, 49 confirmed other neurologic diseases and 25 normal control cases. At the same protein content of 2. 5 pg/ml in 5 antigens, micro-ELISA was done to detect the specific Io-G antibody. The criterion of the positive reaction was the absorbance 0. 18. The results were summarized as follows:
1. In cysticercosis patients, the positive rates of specific IgG antibody were 84.40/6 when cystic fluid was used as antigen; 62.5,1 in scolex antigen, 53. 6% in T. saginata antigen, and 53. 940 in bladder wall antigen. The whole worm extract of C. cellulosae showed the inter-mediate value of the sensitivity, 71. 9%,. The mean absorbance was also the highest in cystic f luid antigen.
2. The non-specific positive reaction was more frequent in T. saginata and scolex antigens than in cystic fluid and- bladder wall antigens.
3. The mean absorbance of cysticercosis
v_
patients to bladder wall antigen was 38.2% of that of cystic fluid antigen; 45.6% in -scolex antigen, 31.7% in T. saginata antigen and 54. 7% in whole worm extract.
4. By disc-PAGE, 6 bands in cystic fluid, 11bands in bladder wall, 9 bands in scolex and 13 bands in T. saginata antigens were recogm zed. Out of them, band C in cystic fluid showed the darkest stainability.
The above results indicated that the sensitive and specific antigenic protein was present in cystic fluid of C. cellulosae the most abundantly. The candidate protein was band C in cystic fluid as seen in the dise-PAGE- but it should be proved.
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